Fig. 6: Serum EVs alter mitochondrial functions in primary rat cortical and midbrain neurons. | npj Parkinson's Disease

Fig. 6: Serum EVs alter mitochondrial functions in primary rat cortical and midbrain neurons.

From: Rotenone induced acute miRNA alterations in extracellular vesicles produce mitochondrial dysfunction and cell death

Fig. 6: Serum EVs alter mitochondrial functions in primary rat cortical and midbrain neurons.

Serum EVs derived from rotenone-treated rats were incubated with primary midbrain ((i) and (ii)) and cortical neurons ((iii) and (iv)), and total cellular ROS was determined by DCFDA, and mitochondrial ROS was assessed using MitoSox staining. UN exo: serum EVs derived from healthy rats, R8 exo: serum EVs derived from rats exposed to rotenone for 8 h, R24 exo: serum EVs derived from rats exposed to rotenone for 24 h. Each independent experiment consisted of a 96-well plate of primary culture, with each well treated with serum EVs from a different animal, resulting in an “n” of 12 per experiment. Five independent experiments were conducted, leading to a total n = 60. Asterisk (*) and (**) indicates values statistically significant from control; p value <0.05 and <0.01 (respectively), SEM of five independent experiments, data were analyzed using one-way ANOVA, followed by Dunnett’s multiple comparisons test.

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