Fig. 9: miR-30a-5p and miR-484 alleviate rotenone-induced toxic effects in SH-SY5Y cells. | npj Parkinson's Disease

Fig. 9: miR-30a-5p and miR-484 alleviate rotenone-induced toxic effects in SH-SY5Y cells.

From: Rotenone induced acute miRNA alterations in extracellular vesicles produce mitochondrial dysfunction and cell death

Fig. 9: miR-30a-5p and miR-484 alleviate rotenone-induced toxic effects in SH-SY5Y cells.

SH-SY5Y cells were transfected with control mimic (CM) and miR-30a-5p or miR-484 mimics and were subsequently treated with 50 nM rotenone. a Total cellular ROS levels were quantified using DCFDA staining, and b mitochondrial ROS levels were assessed by MitoSox staining. The cellular viability was assessed using c MTT assay and d DNA damage ELISA. Asterisk (*), (**), and (****) indicates values statistically significant from control; p value <0.05, <0.01, and <0.0001 (respectively), SEM of eight independent experiments, data were analyzed using two-way ANOVA, followed by Tukey’s multiple comparisons test.

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