Fig. 6: Presence of phosphorylated α-syn in WT mice after CUMS treatment at 15 months of age.
From: Chronic stress induces depression-like behaviors and Parkinsonism via upregulating α-synuclein
WT mice were subjected to 4-week CUMS treatment at 2 months of age. a Representative immunohistochemical results of phosphorylated α-syn in the striatum, hippocampus and substantia nigra at 15 months of age. Scale bar, 20 μm. b Representative immunoblot images of α-syn, phosphorylated α-syn, aggregated α-syn (5G4) and misfolded α-syn (Syn303) in the soluble and insoluble fractions of the striatum. c Representative images showing phosphorylated α-syn (red), P62 (green) and DAPI (blue) staining in the striatum and substantia nigra. Scale bar, 10 μm. CUMS chronic unpredictable mild stress.
