Fig. 6: Long-term efficacy study of SAR446159.

Animals were injected with PFFs at 3 months of age. One week following the PFF administration, animals were randomized and treated with weekly intraperitoneal doses of antibodies for 6 months, a total of 24 doses. The mIgG group was treated with at dose of 15 mg/kg and the non-PFF group was treated with saline. A LB/LN-like inclusions in the ipsilateral SNpc were counted as the average number in 4 fields (2 field per section, 2 sections per animal). B LB/LN-like inclusions in the ipsilateral amygdala (Amy) were counted as the average number in 2 fields (1 field per section, 2 sections per animal). C TH expression in the ipsilateral striatum (STR) was measured as optical density in 2 sections per animal. D Cells expressing p-α-Syn in the ipsilateral cortex were counted as the average number in 6 fields (3 fields per section, 2 sections per animal). E TH-positive cells in both the contralateral and the ipsilateral SNpc were counted as the sum of total TH-positive cells in 9 consecutive sections approximately 120 μm apart per animal. F Latency to fall in rotarod and wire hang tests were averaged over two consecutive testing days. All experiments were performed by investigators blinded to the treatment status. Statistical significance was determined using a one-way ANOVA followed by Dunnett’s multiple comparisons test. Error bars represent the mean ± S.D.