Table 1 SPR parameters

From: A brain-shuttled antibody targeting alpha synuclein aggregates for the treatment of synucleinopathies

 

α-Syn PFF

Capture level (RU)

ka (1/M s) × 106

kd (1/s) × 10−5

KD (pM)

SAR

473 ± 2

4.2 ± 0.2

1.0a

<2.4

1E4

472 ± 3

4.0 ± 0.1

1.0a

<2.5

 

α-Syn monomer

Capture Level (RU)

ka (1/M s) × 106

kd (1/s) × 10−5

KD (pM)

SAR

410 ± 6

1.3 ± 0.1

0.53 ± 0.05

419 ± 60

1E4

537 ± 5

1.5 ± 0.1

0.68 ± 0.04

456 ± 20

  1. Kinetic binding parameters of SAR446159 and 1E4 with α-Syn PFF or monomers. The off rate (kd) for the binding of the α-Syn PFF was below the limit of detection of the instrument and was fixed to the lowest limit for the dissociation time used in the experiment (1.0 × 10−5 1/s). The molarity of α-Syn aggregates was based on a particle size of 1 MDa. As a result, the binding affinities (KD) are estimates. The preferential binding of both molecules to α-Syn PFF over the monomer resulted in a five order of magnitude difference in affinity. All values are the average of n = 3.
  2. akd below the limit of detection.
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