Fig. 2: TH+ neurons of the A11 form direct descending projections to the DHSC.
From: Targeting the hypothalamic a11 nucleus to treat parkinsonian-like nociceptive impairments
A–C Spinal cord coronal sections of DHSC showing CTB+ fibers (A, red), TH+ fibers (B, green) and the merge (C, yellow). D–F Coronal sections of the A11 showing CTB+ neurons (D, red), TH+ neurons (E, green) and the merge (F, yellow). White arrows show the localization of TH+ neurons in the A11 projecting to the DHSC. G Histogram showing the percentage of A11 neurons CTB+/TH+ and CTB+/TH−. H, I Representative examples of coronal sections with TH+ staining at the level of SN and VTA (H) and the same slice with CTB immunostaining (I) showing the absence of projections from the SNc and VTA to the DHSC. Data represented in the histogram are means±S.E.M., Wilcoxon test, ***p < 0.001.
