Table 1 List of antibodies used in the present study

mCherry/rat

mCherry Monoclonal (Clone 16D7)/Cat#M1121 (RRID: AB_253661)

full-length protein

1:500

ThermoFisher (Waltham, MA, USA)

Non-transfected control validated.

Ikui et al.⁹⁶; Zhou et al.⁹⁷; Evans et al.⁹⁸

cfos / guinea pig

c-Fos recombinant monoclonal (clone Gp108B5)/Cat#226 308 (RRID: AB_290559)

residues near the amino terminus

1:1000

Synaptic Systems (Göttingen, Germany)

Vendor-level peptide-competition validation. Synaptic systems, Cat#226308.

human α-syn/mouse

Alpha Synuclein monoclonal (Clone LB 509)/Cat#Ab27766 (RRID: AB_727020)

amino acids 115-122

1:400

ThermoFisher (Waltham, MA, USA)

Validation by epitope mapping.

Jakes et al.⁹⁹

TH / mouse

Tyrosine Hydroxylase Monoclonal (Clone LNC1)/Cat#MAB318 (RRID: AB_2201528)

epitope on the outside of the regulatory N-terminus

1:1000

EMD Millipore (Temecula, CA, USA)

Vendor validated with no cross-reactivity with related hydroxylases and biosynthetic enzymes. EMD Millipore, Cat#MAB318

TH / rabbit

Tyrosine Hydroxylase polyclonal/Cat#P40101-150 (RRID: AB_2617184)

-

1:1000

Pel-Freez (Rogers, AR, USA)

Validated in rat TH studies.

Russo et al.¹⁰⁰

Dopamine receptor 1/rabbit

Dopamine D1R/DRD1 polyclonal/Cat#NBP2-16213 (RRID: AB_2819252)

within the C-terminus region

1:250

Novus Biologicals (Centennial, CO, USA)

Expression validated: Negative tissue (MIHA) + IgG control (WB).

Yan et al.¹⁰¹; Bhowmick et al.¹⁰²

Dopamine receptor 2 / rabbit

DRD2 Polyclonal/Cat#55084-1-AP (RRID: AB_10859941)

-

1:250

Proteintech (Rosemont, IL, USA)

KO/KD validated.

Yan et al.¹⁰³; She et al.¹⁰⁴

DAT / rat

Dopamine Transporter Monoclonal (clone DAT-Nt)/Cat#MAB369 (RRID: AB_2190413)

N-terminus

1:500

Millipore Sigma (Burlington, MA, USA)

KO-validated

Illiano et al.¹⁰⁵

Synaptophysin/rabbit

Synaptophysin Polyclonal/Cat#PA1-1043 (RRID: AB_2199026)

amino acids 253-272

1:200

Invitrogen (Waltham, MA, USA)

Vendor validated-Tissue-specific WB (neural positive, non-neural negative); cross-species verified (Invitrogen, Cat#PA1-1043).

Alexa Fluor 488 anti-rabbit (H + L)/ goat

Alexa Fluor® 488 goat anti-rabbit/Cat#A-11008 (RRID: AB_143165)

1:1000

Invitrogen (Waltham,

MA, USA)

Alexa Fluor 488 anti-mouse (H + L)/goat

Alexa Fluor® 488 goat anti-mouse/Cat#A-11029 (RRID: AB_2534088)

1:1000 (in vivo);

1:800 (in vitro)

Invitrogen (Waltham,

MA, USA)

Alexa Fluor 488 anti-guinea Pig IgG (H + L)/donkey

Alexa Fluor® 488 AffiniPure® Donkey anti-Guinea Pig IgG (H + L)/Cat#706-545-148 (RRID: AB_2340472)

1:250

Jackson ImmunoResearch Laboratories Inc (West Grove, PA, USA)

Alexa Fluor 568 anti-rat (H + L)/goat

Alexa Fluor® 568 anti-rat/Cat#11077 (RRID: AB_2534121)

1:1000

ThermoFisher (Waltham, MA, USA)

Alexa Fluor 633 anti-rabbit (H + L)/goat

Alexa Fluor® 633 anti-rabbit/Cat# A-21071 (RRID: AB_2535732)

1:250 (in vivo – DRD1 and DRD2 staining); 1:400 (in vivo – synaptophysin staining); 1:500 (in vivo – DAT staining);

1:800 (in vitro)

Invitrogen (Waltham, MA, USA)

Cyanine5/goat

Cy™5 AffiniPure® Goat Anti-Rat IgG (H + L)/Cat#112-175-143 (RRID: AB_2338263)

1:500

Jackson ImmunoResearch Labs (West Grove, PA, USA)