Fig. 5: Elongated mitochondria in Mff knockdown axons exhibit increased Ca2+ uptake upon stimulation. | npj Parkinson's Disease

Fig. 5: Elongated mitochondria in Mff knockdown axons exhibit increased Ca2+ uptake upon stimulation.

From: Systematic evaluation of mitochondrial morphology regulators for amelioration of neuronal α-synucleinopathy

Fig. 5: Elongated mitochondria in Mff knockdown axons exhibit increased Ca2+ uptake upon stimulation.The alternative text for this image may have been generated using AI.

a Representative images of axonal mito-jGCaMP8m fluorescence at peak intensity following stimulation (10AP at 10 Hz) in control, α-Syn, α-Syn + shMff and α-Syn + shFis1 groups. b Integrated intensity of mito-jGCaMP8m signals from full-length mitochondria associated with single presynaptic sites. c Quantification of area under the curve (AUC) shows enhanced mitochondrial Ca2+ uptake in the Mff knockdown group. One-way ANOVA, **p = 0.004, ###p < 0.0001, #p = 0.012 (for α-Syn + shMff), #p = 0.045 (for α-Syn + shFis1), n.s. not significant. nControl = 21 images, nα-Syn = 23 images, nα-Syn+shMff = 16 images, nα-Syn+shFis1 = 20 images. Samples were obtained from 6 independent experiments from different pups. Number of mitochondria are annotated at the bottom of bar graphs. Scale bar = 2 μm.

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