Fig. 5: Topical treatment with PE-O4 HDL NPs reduces corneal haze and inflammation during the acute phase of NM injury. | npj Regenerative Medicine

Fig. 5: Topical treatment with PE-O4 HDL NPs reduces corneal haze and inflammation during the acute phase of NM injury.

From: A novel therapy to ameliorate nitrogen mustard-induced limbal stem cell deficiency using lipoprotein-like nanoparticles

Fig. 5

Two hours post NM exposure, corneas were treated topically with PE-O4 HDL NP, and PBS (control) once daily for 3 days. A Clinical images visualized corneal haze. PE-O4 HDL NPs attenuates NM-induced corneal haze score. N = 15. B H&E staining was conducted. Then, immune cell filtration throughout the stroma and epithelial thickness was quantified using ImageJ. PE-O4 HDL NPs reduced immune cell infiltration in corneal stroma and prevented epithelial thinning 3 days post-NM exposure (acute phase). N = 5. C Three days post exposure, RT-qPCR was conducted to examine the expression of pro-inflammatory genes in mouse corneas. PE-O4 HDL NPs reduced the levels of proinflammatory gene expression induced by NM exposure during the acute phase. N = 10. Experiments were repeated two times. p values are indicated at the figure.

Back to article page