Fig. 3: Db/db mouse wounds exhibit perturbed lipid homeostasis, which can be rescued by topical cyclodextrin application.

a Venn diagram displaying number of genes in common between wounds harvested at day 5 vs day 0 from db/+ mice (I), wounds harvested from db/db vs db/+ mice at day 5 (II), and wounds harvested at day 5 from db/db mice that were topically treated with Methyl-β-cyclodextrin (MβCD) vs vehicle (III); b Heatmap of functions and pathways exhibiting directionality based on z-scores. z-score represents the predicted directionality of the enriched pathways or functions (z-score ≥ 2 is considered as induced). Network analysis of shared genes associated with fatty acid metabolism between c day 5 vs day 0 from db/+ mice and d wounds harvested at day 5 from db/db mice that were topically treated with MβCD vs vehicle, and comparing with e wounds harvested from db/db vs db/+ mice at day 5. Green – decreased gene expression; red – increased gene expression; gray – not regulated; blue – predicted suppression; orange – predicted activation. f Validation of gene expression levels by qRT-PCR [n = 3 wounds, plotted as means with error bars corresponding to standard deviation, *p < 0.05; ****p < 0.0001; Two-way Analysis of Variance followed by Tukey’s multiple comparison testing].