Fig. 4: Topical cyclodextrin disrupts cholesterol synthesis and transport in vivo diabetic mouse wounds and human skin ex vivo. | npj Regenerative Medicine

Fig. 4: Topical cyclodextrin disrupts cholesterol synthesis and transport in vivo diabetic mouse wounds and human skin ex vivo.

From: Scaffolds with spatiotemporally controlled growth factor delivery and cyclodextrin-enabled antagonism of growth factor receptor sequestration promote cutaneous wound healing

Fig. 4

a Filipin staining confirms reduced free cholesterol levels in MβCD treated mouse wounds (scale bar = 25 µm). b Total cholesterol levels from diabetic mouse wounds (including free cholesterol and cholesterol esters) were quantified by fluorometry and found to be diminished in MβCD treated mouse wounds [n = 9 from three technical replicates × three biological replicates, plotted as means with error bars corresponding to standard deviation]. c Representative images of ToF-SIMS analysis demonstrating penetration of cyclodextrin through multiple layers of the epidermis to diminish total cholesterol levels in healthy human skin.

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