Fig. 3: Single nucleus RNA sequencing (snRNA-Seq) reveals diverse aberrant epithelial and mesenchymal cell phenotypes in bleomycin-injured ferret lungs.
From: Ferret model of bleomycin-induced lung injury shares features of human idiopathic pulmonary fibrosis
A Schema showing workflow for generating and analyzing lung snRNA-Seq data (created with BioRender’s icons). B UMAP of major cell classes (epithelial, endothelial, mesenchymal, and immune) captured in snRNA-seq analysis. C UMAP of all cells profiled; the 33 clustered cell types were annotated using human cell reference annotations from the HCLA. D UMAP showing the distribution of cell-types in bleomycin-injured (blue) and saline-treated (orange) ferret lungs. Heatmap of marker genes used for classifying epithelial (E) and mesenchymal (F) cell types. Each cell type is represented by the top 5 genes ranked by Wilcoxon rank sum test for each cell type against the other cell types.
