Fig. 7: Early treatment of hyperglycemia restores the force generating capacity of muscle stem cell derived 3D cultures.
From: Intrinsic muscle stem cell dysfunction underlies functional deficits in models of type 1 diabetes
a Representative brightfield and yellow fluorescent protein (YFP) images of end-point 3D cultures derived from isolated muscle stem cells from wildtype (WT, gray) and diabetic Ins2 (Akita, red) mice treated with vehicle (veh) or dapagliflozin (dapa) (scale bar = 500 μm). b Quantification of the tetanic force-producing capacity of micromuscles (n = 3–4 mice per treatment group). Quantification of (c) myotube diameter (n = 3–4 mice per treatment group) and (d) myotube fusion index. e Representative images of differentiated myotubes from isolated muscle stem cells of veh or dapa treated WT and Akita mice stained with myosin heavy chain (MHC, green) and DAPI (blue) (scale bar = 50 μm). Statistics: Two-way ANOVA with Bonferroni’s post-hoc test *P < 0.05. Data are mean ± SD.
