Fig. 3: Cytotoxicity and antibacterial activity of hydrogels. | npj Regenerative Medicine

Fig. 3: Cytotoxicity and antibacterial activity of hydrogels.

From: Novel copper-ion coordinated andrographolide-loaded hydrogel activates Rac1/JNK1 axis for enhancing diabetic wound healing

Fig. 3: Cytotoxicity and antibacterial activity of hydrogels.

A, B Viability of HaCaT keratinocytes (A) and HFF-1 fibroblasts (B) exposed to hydrogel extracts for 6-24 h (n = 3). C, D Representative graphs of cell scratch assay of hydrogels acting on glycosylated HaCaT cells and HFF-1 cells. Scale Bar: 50 μm. E, F Statistical graphs of migration rates of glycosylated HaCaT cells and HFF-1 cells (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001, vs Control group, #P < 0.05, ##P < 0.01, ###P < 0.001, vs Matrix group. G Representative inhibition zones graphs of hydrogel against E. coli and S. aureus (n = 3). Scale Bar: 1 cm. ***P < 0.001, vs Blank group, #P < 0.05, vs Matrix group. H, I Quantification of bacteriostatic activity against E. coli (H) and S. aureus (I) by plate colony counting assay (n = 3). J, K Quantification of inhibition zone diameters for E. coli (J) and S. aureus (K) (n = 3). The bar charts present mean ± SD. ***P < 0.001, vs Matrix group, ##P < 0.01, ###P < 0.001, vs Matrix group.

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