Fig. 6: In vivo wound healing and perfusion assessment in a diabetic mouse model.

A Schematic timeline of the in vivo wound healing experiment. Full-thickness skin wounds with a diameter of 0.8 cm were created on both sides of the dorsal midline. The day of surgery was designated as day 0. Treatments were initiated from day 1 onwards. The schematic indicates the time points for RFLSI monitoring, photographic documentation, and sample collection throughout the 12-day study period. B Representative images of wounds in different treatment groups on days 0, 3, 7, and 12. Grid marks at the bottom indicate 1 mm divisions. C Wound closure rates for each group over 12 days. The graph visually represents changes in wound area over time. Scale Bar: 2 mm. D Wound healing rate. E Representative RFLSI perfusion maps of wound regions at days 3, 7, and 10, with red and blue colors indicating high and low blood flow, respectively. Scale Bar: 5 mm. F Quantitative analysis of perfusion signal intensity within the wound area over time. The bar charts present mean ± SD (n = 6 per group). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, vs Control group, ^#P < 0.05, ^###P < 0.001, vs Matrix group.