Fig. 2: LMNA (K117fs) hiPSC and hiPSC-CMs exhibit shortened telomeres.

A Pt3, part of a three-generation family that had 15 affected members exhibiting autosomal dominant cardiac disease, used for the generation of hiPSC. Square, males; circles, females; slash, deceased. Pedigree originally reported in Heart Rhythm 2009 May; 6(5): 707–710. B Generation of hiPSC lines and subsequent hiPSC-CM differentiation. Representative micrographs of LMNA + / + , +/mut, mut/mut, and mut/null hiPSC-CMs stained with cardiac troponin cTnT (green), TelC telomeres (red), and nuclei (DAPI) are shown. C Telomere levels of hiPSC and (D) hiPSC-CMs were quantified. Data are shown as violin plots where blue median and gray quartiles are shown. One-way ANOVA tests by comparing the mean of each group with the mean of every other group, followed by Holm–Sídák multiple comparison test were used.