Fig. 5: CLSM images of single-species and dual-species biofilms formed by S. aureus and P. fluorescens after 24, 48, and 72 h of incubation, following SYBR Green staining.

The 3D CLSM images of dual-species biofilms cultured for 48 h. SYBR Green was employed to stain biofilm distribution. Scale bars represent 100 μm. (SA stands for S. aureus biofilm group, PF stands for P. fluorescens biofilm group, and SP stands for biofilm formed by co-culture).