Fig. 6: Analysis of CLSM parameters of single- and dual-species biofilms by incubation for 24, 48, and 72 h.

a Biofilm roughness. b Biofilm thickness. c Biofilm volume; d Porosity. Analysis of the inhibition rate of CD and QAC on biofilms formed by S. aureus and P. fluorescens for 48 h: e the activity of cell, f the biomass of biofilm (Control Check (CK) refers to the group that has not been treated with disinfectants, SA stands for S. aureus biofilm group, PF stands for P. fluorescens biofilm group, and SP stands for biofilm formed by co-culture). Note: different letters (a, b, c) indicate significant differences in the treatment groups (p < 0.05).