Table 2 Genes related to S. aureus and P. fluorescens biofilm
bacteria | Genes | Sequences (5’ → 3’) | Function | Ref. |
|---|---|---|---|---|
S. aureus | 16S rRNA-F | AGCGGTAAACACAATTCACTTAACAC | Housekeeping | |
16S rRNA-R | CACGATAGAGCAGAAACATTTGGTAAG | |||
icaA-F | AGCAGTTGTACTGGTCGCATTTCC | Intercellular adhesion | ||
icaA-R | ACGTTGGGTATTCCCTCTGTCTGG | |||
icaB-F | TTGAAACACATACCCACGATTTGC | |||
icaB-R | CATTGGAGTTCGGAGTGACTGC | |||
icaD-F | TCAAGCCCAGACAGAGGGAATACC | |||
icaD-R | GCGACAAGTGCTGTTTCTCTAACG | |||
P. fluorescens | 16S rRNA-F | GGTTGTAAGCCTTGTTCCATTTGATC | Housekeeping | |
16S rRNA-R | CACGCCGAAGAGTTATCCACAG | |||
flgA-F | TGATTCGCAAGGGTGACCAAGTG | Flagella | ||
flgA-R | TTGACACGGATCTGTTCGCTCATG | |||
lapD-F | TGATTCGCAAGGGTGACCAAGTG | Regulate surface attachment | ||
lapD-R | CGCTTCCTGCCTTGGCTTGAG | |||
pgaC-F | TGCAGGTCGGCGAGTTCTCC | Essential for the adhesin | ||
pgaC-R | GATGCGGTCCAGGGCTTTCTTG | |||
pgaA-F | TACGGTGGCGGCAATAACAACG | Exporting the biofilm adhesin across the outer membrane | ||
pgaA-R | GAAGTCGGCGGTGGCATAGC | |||
pgaD-F | GAAGAAGGCGGCGTCGAAGAAG | Assist the glycosyltransferase pgaC in the polymerization of PGA | ||
pgaD-R | TTGTCGGGCACCAGGGTCAG |
