Fig. 4: Effect of Reb Aα on HFD-induced steatosis and thermogenesis.

A mRNA levels of G6pc (left) and Pck1 (right) in the liver (n = 6 per group). B Glucose tolerance test (left) and AUC (right) (n = 10 per group). C Fasting glucose level (left, n = 10 per group) and glucose level under feeding state (right, n = 5 per group). (D) mRNA levels of the indicated genes in the liver (n = 6 per group). E Immunoblot of total liver lysates (left) and quantification of protein levels (right) (n = 4 per group). F Liver weight (n = 10 per group). G Hematoxylin and eosin (H&E) staining of the liver (n = 5 per group). Representative images are shown. Scale bar = 100 μm. H Serum ALT and AST levels (n = 5 per group). I Brown adipose tissue (BAT) weight (n = 10 per group). J Hematoxylin and eosin (H&E) staining of BAT (n = 5 per group). Representative images are shown. Scale bar = 100 μm. K mRNA levels of the indicated genes in BAT (n = 6 per group). L Immunoblot of total BAT lysates (left) and quantification of protein levels (right) (n = 4 per group). All values are presented as the mean ± SEM. Statistical analysis was conducted using repeated two-way ANOVA (B [left]) and one-way ANOVA (A, B [right], C–L). Asterisks exhibit significant differences: *p < 0.05; N.S., not significant.