Fig. 5: Analysis of the structure of plant-derived scaffolds and the green onion scaffold seeded with CMSCs. | npj Science of Food

Fig. 5: Analysis of the structure of plant-derived scaffolds and the green onion scaffold seeded with CMSCs.

From: Chick embryo extract-driven culture system enhances chicken muscle satellite cell differentiation and 3D myotube formation

Fig. 5

a Schematic of the plant-derived scaffold fabrication process. b FE-SEM images of plant-derived scaffolds. Scale bars, 100 μm. c FE-SEM images of the green onion scaffold seeded with CMSCs. Scale bars, 20 μm (above)/10 μm (below). d Live/dead staining images of CMSCs seeded on green onion scaffold. Representative images were selected from areas enriched with attached cells. Scale bars, 100 μm. e Representative immunofluorescence images of CMSCs seeded on green onion scaffold. Cell nuclei were stained with DAPI (blue) and myotubes were stained with phalloidin (green) and skeletal muscle differentiation marker MYHC (red). Scale bars, 25 μm.

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