Fig. 3: Integrated assessment of GFE-EA derived from the SY-PCS IFS on B16-F10 cells and zebrafish. | npj Science of Food

Fig. 3: Integrated assessment of GFE-EA derived from the SY-PCS IFS on B16-F10 cells and zebrafish.

From: Sustainable repeated-batch fermentation of immobilized Ganoderma formosanum for enhanced production of anti-melanogenic bioactive compounds

Fig. 3

Cell viability of B16-F10 cells treated with different concentrations of GFE-EA (obtained from the SY) for 24 h (A) and 48 h (B). Intracellular melanin content in B16-F10 cells following GFE-EA treatment (C). Effect of GFE-EA on zebrafish heart rate (D). Zebrafish melanogenesis assays, including tyrosinase activity (E) and melanin content (F). Phenotypic observations of zebrafish under GFE-EA treatment (G). All data are expressed as mean ± SD (n ≥ 3). Different letters indicate significant differences according to Duncan’s multiple range test (p < 0.05). NC Negative control, KA Kojic acid (100 µg/mL); SY-PCS contains PP, dried ground soybean hulls, and yeast extract, GFE-EA Hexane fraction of GFM.

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