Fig. 5: Effect of medium pH on fed-batch heme production by Saccharomyces cerevisiae KCCM 12638.
From: Enhanced heme production in industrial Saccharomyces cerevisiae through metabolic engineering
Fed-batch heme production using the Saccharomyces cerevisiae KCCM 12638 strain at pH 5.5 (A), pH 4.5 (B), and pH 3.5 (C). After the initially added glucose was depleted, a feeding solution containing 350 g/L glucose, 280 g/L yeast extract, and 140 g/L peptone was added to the bioreactor (black dot arrow). The detailed data are presented in Supplementary Fig. 4. Throughout the cultivation period, the medium temperature, agitation speed, and aeration rate were maintained at 25 °C, 700−1200 rpm, and 2.0−5.0 vvm, respectively. Measurements were performed in duplicate, and average values with standard deviations of less than 5% are presented.
