Fig. 7: Effect of dietary perennial ryegrass on key signaling pathway and their genes improving meat quality.
From: Dietary perennial ryegrass improves meat quality: insights into the gut-muscle axis in geese
a–f AMPK (AMP-activated protein kinase), FOXO3 (forkhead box O3), Akt (protein kinase B), mTOR (mammalian target of rapamycin), P70S6K (ribosomal protein S6 kinase), and 4EBP,1 respectively. g–k Western blot of Akt, mTOR, AMPK, and FOXO3, respectively. l Immunofluorescence (IF) images of AMPK, Akt, and mTOR. Rabbit anti-AMPK (1:500/1:200) showing nuclear translocation of AMPK. Blue: nucleus (DAPI); Red: AMPK staining; Merge; combination of blue and red indicating nuclear translocation of AMPK, scale bar = 200 μm. Immunofluorescence (IF) analysis using Rabbit Anti-Akt (1:500/1:200) showing nuclear translocation of Akt in the breast muscle tissue of geese. Blue: nucleus (DAPI); Red: Akt staining; Merge; combination of blue and red indicating nuclear translocation of Akt, scale bar = 200 μm. Immunofluorescence (IF) analysis using Rabbit Anti-mTOR (1:500/1:200) showing nuclear translocation of mTOR in the breast muscle tissue of geese. Blue: nucleus (DAPI); Red: Akt staining; Merge; combination of blue and red indicating nuclear translocation of mTOR, scale bar = 200 μm. m Correlation between key signaling genes and meat quality indicators.
