Fig. 2: Construction of HepG2 3D platforms.

A Expansion of HepG2 cells. Aa: Microscopic images of HepG2 2D cells; Ab: Physical picture of HepG2 2D cell culture dish; Ac: Growth time microscopy images of 3D liver organoid. B Construction of HepG2 3D liver organoids. Ba: U-type ultra-low adsorption 96-well plate physical picture; Bb: 3D organoid culture physical drawing; Bc: Growth time microscopy images of 3D liver organoid; Bd: Confocal fluorescence images of 3D liver organoids. C Construction of HepG2 Livers-on-a-Chip. Ca: Schematic diagram of livers-on-a-chip cell culture process; Cb: Physical drawing of the liver chip microfluid system; Cc: Confocal fluorescence images of liver chip.