Table 2 The retention time, linearity, limit of detection, and limit of quantification of 27 antimicrobial substances in solvent (water:acetonitrile, 50:50, v/v) and soybean extracted solution

From: A novel LC-MS/MS multi-group method for simultaneous determination of antimicrobial residues in legume-based alternative proteins

No

Analyte

RT

In solvent

In matrix

Range (ng.mL–1)

Equation

R2

LOD(ng.mL–1)

LOQ (ng.mL–1)

Range (ng.g–1)

Equation

R2

LOD (ng.g–1)

LOQ (ng.g–1)

1

BCT

8.01

50–500

y = 15.536x - 578.78

0.9925

17.81

53.96

50–200

y = 17.687x + 24.789

0.9917

15.64

47.40

2

CBD

3.93

25–200

y = 35.617x + 1383

0.9929

4.75

14.38

10–100

y = 80.249x + 1266

0.9765

2.11

6.38

3

CFZ

7.44

10–500

y = 18.683x + 256.17

0.9972

6.20

18.80

10–200

y = 36.091x - 18.355

0.9993

3.21

9.73

4

CPR

8.03

1–500

y = 194.15x + 1324.9

0.9984

0.48

1.46

2.5–200

y = 85.658x - 137.25

0.9997

1.09

3.31

5

DCX

8.23

5–500

y = 36.703x + 29.419

0.9999

3.21

9.73

5–200

y = 42.275x + 2.4354

0.9998

2.79

8.45

6

DFN

9.38

0.5–500

y = 2081.5x + 5169

0.9970

0.17

0.52

0.5–200

y = 5538.6x - 2143.5

0.9997

0.06

0.20

7

DXC

8.10

2.5–500

y = 1083.8x + 9107.9

0.9988

9.38

28.43

2.5–200

y = 7847.1x + 6247.8

0.9992

1.30

3.93

8

ERF

7.53

1–500

y = 3833.5x + 32778

0.9980

0.15

0.47

1–200

y = 8690.2x - 8251.3

0.9996

0.07

0.21

9

ETM

8.19

0.5–500

y = 1436.4x + 11698

0.9977

0.25

0.76

0.5–200

y = 4266x + 11017

0.9985

0.08

0.26

10

FCN

7.89

1–500

y = 377.37x + 267.68

0.9999

0.58

1.77

0.5–200

y = 947.15x - 837.07

0.9998

0.23

0.70

11

FTD

7.39

2.5–500

y = 1232x + 12929

0.9974

0.47

1.44

2.5–200

y = 1486.1x + 5011.8

0.9978

0.39

1.19

12

FZD

7.71

50–500

y = 35x + 1018.2

0.9956

7.50

22.73

50–200

y = 19.272x + 117.72

0.9810

13.62

41.27

13

FA

10.13

50–500

y = 12.77x + 14.286

0.9994

6.99

21.18

50–200

y = 22.082x - 155.75

0.9970

4.04

12.25

14

LCM

7.49

0.5–500

y = 27246x + 335733

0.9954

0.04

0.12

0.5–200

y = 28193x + 24561

0.9994

0.04

0.12

15

MG

8.16

0.5–500

y = 14001x + 34324

0.9997

0.16

0.48

0.5–200

y = 16838x - 55345

0.9972

0.13

0.40

16

MCN

7.67

0.5–500

y = 19526x + 271142

0.9940

0.04

0.14

0.5–200

y = 19322x + 441.68

0.9998

0.05

0.14

17

OTC

7.73

25–500

y = 272.55x + 6592.2

0.9931

1.62

4.91

25–200

y = 559.14x + 918.36

0.9993

0.79

2.39

18

PenG

8.07

25–500

y = 112.85x + 915.56

0.9988

15.61

47.30

25–200

y = 583.64x + 896.83

0.9992

3.02

9.15

19

PRC

7.90

25–500

y = 242.4x + 565.67

0.9999

1.57

4.74

2.5–200

y = 659.53x - 261.41

0.9997

0.58

1.74

20

RFX

8.79

5–500

y = 182.56x + 317.61

0.9999

1.02

3.09

5–200

y = 466.48x - 1595.4

0.9986

0.40

1.21

21

STM

3.43

25–500

y = 203.29x - 7268

0.9930

0.94

2.85

n.d.

n.d.

n.d.

n.d.

n.d.

22

SFD

7.36

5–500

y = 1054.9x + 20021

0.9950

0.65

1.98

5–200

y = 2579.2x + 22860

0.9943

0.27

0.81

23

TCN

8.75

2.5–500

y = 569.95x + 179.32

1.0000

0.18

0.56

0.5–200

y = 2579.2x + 22860

0.9972

0.07

0.20

24

TPN

7.58

2.5–500

y = 99.87x + 845.5

0.9978

1.00

3.03

2.5–200

y = 43.937x + 65.881

0.9995

2.27

6.88

25

TML

8.13

0.5–500

y = 14300x + 204831

0.9933

0.05

0.15

0.5–200

y = 21410x - 5435

0.9998

0.03

0.10

26

VCM

6.93

25–500

y = 17.234x + 47.52

0.9991

4.67

14.15

25–200

y = 14.874x - 34.941

0.9993

5.41

16.40

27

VGM

8.63

10–500

y = 43.63x - 94.741

0.9998

2.50

7.59

10–200

y = 110.09x - 284.78

0.9976

0.99

3.01

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