Fig. 5: Evolutionary dynamics and genomic characterization of the pSK41-carrying clade within CC5 lineages.

A Time-scaled phylogenetic tree of bacterial isolates showing the presence (teal) or absence (black) of pSK41-family plasmids in subclade CC5.6. Each terminal node represents a single isolate, with color indicating whether a pSK41-family plasmid was identified; B Circular comparison of plasmid sequences against the reference plasmid pSK41. The innermost ring shows the coding sequences (CDSs) of reference pSK41 (NC_005024.1). GC skew is displayed in green (positive) and purple (negative) in the second rings. The outer rings represent alignments of other plasmids (labeled by GenBank accession numbers) with pSK41, with colored regions indicating conserved sequences. Blue gene labels (e.g., rep1, traO, bleO, aadD, mobV) denote the difference between the plasmids in clade CC5.6 and the reference.