Fig. 5: LYC alleviates T-2 toxin-induced hepatic ferroptosis by activating Nrf-2 pathway. | npj Science of Food

Fig. 5: LYC alleviates T-2 toxin-induced hepatic ferroptosis by activating Nrf-2 pathway.

From: Lycopene mitigates T-2 toxin-induced hepatic ferroptosis by targeting the Nrf2/mitophagy axis in mice

Fig. 5: LYC alleviates T-2 toxin-induced hepatic ferroptosis by activating Nrf-2 pathway.The alternative text for this image may have been generated using AI.

A 3D structure docking simulation of LYC-Nrf2. B 2D binding sites of LYC-Nrf2. C 3D structure docking simulation of LYC-Keap1. D 2D binding sites of LYC-Keap1. (E) A schema diagram of LYC treatment model by giving LYC daily for 28 days to C57 mice. F Mice weight, liver coefficient, AST activity, ALT activity and Ishak score. G Representative images of liver autopsy photos, HE stained liver section and ROS fluorescence results (Scale bar = 50 µm). Red triangle: inflammatory cell infiltration, Yellowe triangle: nuclear condensation, Blue triangle: cellular vacuolization. H Liver iron content, GPx-4 activity, MDA and 4-HNE level from 3 individual samples. I Nrf2 protein expression normalized by GAPDH using total liver protein, and Nu-Nrf2 normalized by Histone using liver nuclear protein present in bar graph with representative images of western blot results. J Heatmap shows the quantification of Nrf2 related gene expression in Nrf2 activation model. K Representative images of immunofluorescence experiments stained for FTH, Nrf2 and LC3-Parkin. L, M Mitophagy and ferroptosis related protein expression, total protein normalized by GAPDH, mitochondrial protein expression normalized by COX IV. Error bars are standard deviation (SD) (n = 3). Statistical analysis was done using one-way ANOVA; *, P ≤ 0.05; **, P ≤ 0.001 comparing to CG group, #, P ≤ 0.05; ##, P ≤ 0.001 comparing to T-2 group.

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