Fig. 5: Circadian rhythms of hair follicles in a temperature-controlled chamber.
From: Low temperature abolishes human cellular circadian rhythm through Hopf bifurcation
A Hair follicle collection procedure. Six participants arrived at the experimental facility at 19:00 and were dressed in experimental clothes after receiving brief instructions for the experiment. The participants stayed in the isolated temperature-controlled chamber (28 °C and 18 °C) until the end of the experiment. Ten hair follicles were collected from participants at 22:00, 4:00, 10:00, 16:00, and 22:00. The hair follicle samples were immediately soaked in RNAlater and stored at −70 °C until RNA analysis. B Scalp skin temperature under different chamber temperatures. Each participant’s scalp skin temperature was measured three times at one-minute intervals. The value is presented as the mean ± SD. C Expression profiles of the circadian clock gene under different temperatures. The error bars indicate the SD of three repeated measurements by real-time qPCR. D Circadian amplitude of gene expression profiles under different temperatures. Each dot represents the circadian amplitude of hair follicle cells from an individual participant, shown as the SD. Gray lines connect the amplitudes of the same participant under different temperature conditions.
