Fig. 1: Schematic of the analytical workflow.
From: Mapping fusion-driven cell reprogramming through integrative single-cell computational frameworks
The investigation comprised three key phases: (1) Unsupervised clustering and TI were applied to the co-culture scRNA-seq datasets. (2) Intercellular communication patterns were analyzed to compare interactions between mMSC, mHL1, and their fused hybrid cell populations at both Day 1 and Day 3. (3) Intracellular GRNs were inferred to characterize the transcriptional reprogramming events associated with cell fusion across the Day 1 and Day 3 time points.
