Fig. 3: Immune protection effect of G mRNA-LNPs against IHNV infection. | npj Vaccines

Fig. 3: Immune protection effect of G mRNA-LNPs against IHNV infection.

From: Development of an mRNA vaccine encoding IHNV glycoprotein protects rainbow trout (Oncorhynchus mykiss) from infection

Fig. 3

A Scheme of vaccination and challenge (Created with BioRender.com with permission). Rainbow trout (n = 40 per group) were immunized with 10 μg doses of mG-LNPs. The control groups received PBS or 10 μg doses of FLUC-LNP. At 14 dpv and 28 dpv, serum from rainbow trout was collected to detect IgM titers (n = 6) and neutralizing antibody levels (n = 4). At 28 dpv, rainbow trout (n = 30 per group) were challenged with 103 TCID50 GS21 virus and monitored for survival rates for 14 days. B Serum IgM antibody levels at 14 dpv and 28 dpv (n = 6). C Serum neutralizing antibody levels at 14 dpv and 28 dpv (n = 4). D Survival rates of vaccinated rainbow trout. Survival curves are from two independent experiments. E RPS of vaccinated groups and negative groups after challenge. All data were presented as mean ± SEM and analyzed by two-way ANOVA (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns, not significant).

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