Fig. 9: ELISA analysis of specific serum antibodies in sheep.

A, B show responses of Haemonchus-specific IgG and IgE antibodies, respectively. Assays were performed using plates coated with soluble proteins of adult parasites and combined sheep sera. C, D illustrate antigen-specific IgG and IgE responses in individual sheep. Vaccine-specific antibodies for the GEA and NEA groups were measured using plates coated with an equal amount of the corresponding vaccine antigens (marked with white arrows); the BVAX and negative and positive control groups were assayed using Barbervax®-coated plates. Data are plotted on different y-axis scales to better visualize the pattern of the different assays.