Fig. 4: Dose-dependent cellular immune response to HPV-mRNA-poly(A). | npj Vaccines

Fig. 4: Dose-dependent cellular immune response to HPV-mRNA-poly(A).

From: Loop structure in poly(A) tail of mRNA vaccine enhances antigen translation efficiency and mRNA stability

Fig. 4

A HPV-mRNA-poly(A) at doses of 3 μg and 10 μg was administered twice to 6-week-old female C57BL/6 mice (n = 5). The nil group was treated with DPBS. One week after the last administration, splenocytes were harvested for flow cytometry analysis. B Antigen-specific CD8 + T-cells in splenocytes without peptide stimulation. C Cytokine (Interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha) production and D T cell activation markers in splenocytes stimulated with peptides. Data are presented as mean values ± SD. P values were calculated using a two-way ANOVA. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

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