Fig. 3: Immunization with NDV and MVA viral vectors encoding SARS-CoV-2 S protein induce systemic and mucosal SARS-CoV-2-specific humoral immune responses in K18-hACE2 transgenic mice challenged with SARS-CoV-2. | npj Vaccines

Fig. 3: Immunization with NDV and MVA viral vectors encoding SARS-CoV-2 S protein induce systemic and mucosal SARS-CoV-2-specific humoral immune responses in K18-hACE2 transgenic mice challenged with SARS-CoV-2.

From: Immunogenicity and efficacy of homologous and heterologous NDV and MVA SARS-CoV-2 vaccines in mice and hamsters

Fig. 3

A Systemic anti-S IgG titers against Wuhan determined by ELISA in individual mouse serum samples collected from K18-hACE2 mice at day 14 post-prime, day 26 post-boost, day 5 post-challenge and day 17 post-challenge. The geometric mean with geometric SD of endpoint titers from duplicates of each sample for each group are represented. The dotted line represents the LOD. Unpaired ordinary one-way ANOVA followed by Tukey’s multiple comparisons test of transformed data: *p < 0.033; **p < 0.002; ***p < 0.0002; ****p < 0.0001. B Isotype anti-S IgG1, IgG2c and IgG3 titers against Wuhan determined by ELISA in pooled mouse serum samples (n = 5) collected from K18-hACE2 mice at day 26 post-boost. The geometric mean with geometric SD of endpoint titers from duplicates of each sample for each group are represented. The dotted line represents the LOD. Two-way ANOVA followed by Tukey’s multiple comparisons test: *p < 0.033; ***p < 0.0002; ****p < 0.0001. C Systemic anti-S IgG titers against Wuhan, Beta, Omicron BA.1, BA.5 and XBB.1.5 determined by ELISA in pooled mouse serum samples (n = 5) collected from K18-hACE2 mice at day 14 post-prime and day 26 post-boost. The geometric mean with geometric SD of endpoint titers from duplicates of each sample for each group are represented. The dotted line represents the LOD. Two-way ANOVA followed by Tukey’s multiple comparisons test: *p < 0.033; ****p < 0.0001. D Neutralizing antibody titers against SARS-CoV-2 Wuhan (MAD6 isolate). NT50 titers were evaluated in individual mouse serum samples collected at day 26 post-boost, day 5 post-challenge and day 17 post-challenge using a live virus MNT assay. The geometric mean with geometric SD of NT50 values from triplicates of each sample for each group are represented. The dotted line represented the LOD. Unpaired ordinary one-way ANOVA followed by Tukey’s multiple comparisons test of transformed data: *p < 0.033; **p < 0.002; ***p < 0.0002. E SARS-CoV-2 neutralizing antibody titers against several SARS-CoV-2 variants of concern. NT50 titers against Wuhan (MAD6 isolate), Beta, Omicron BA.1, BA.5 and XBB.1.5 were evaluated in pooled mouse serum samples (n = 5) collected at day 26 post-boost, using a live virus MNT assay. The geometric mean with geometric SD of NT50 values from triplicates of each pooled group sample are represented. The dotted line represents the LOD. Unpaired ordinary one-way ANOVA followed by Tukey’s multiple comparisons test of transformed data: *p < 0.033; ****p < 0.0001. F, G Mucosal anti-S IgA (F) and anti-S IgG (G) levels against Wuhan determined by ELISA in pooled NW (n = 5) collected from K18-hACE2 mice on day 17 post-challenge. For the PBS/PBS group, pooled NW were collected on day 5 post-challenge. Data are presented as mean optical density (OD) at 450 nm from serial dilutions, with SD calculated from duplicate measurements.

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