Table 2 List of qPCR primers and probes
Target region | Primer sequence (5’→ 3’) | Product size (bp) |
|---|---|---|
SPIKE 1A | Forward: GGC AAG GGC TAC CAC CTG ATG AG | 228 |
Reverse: TGT TGT CGG TGG TGA TGA TCT GG | ||
SPIKE 1B | Forward: GGC AAG GGC TAC CAC CTG ATG AG | 108 |
Reverse: TGG RGC GGT GGT GAA RTT CTT CTC | ||
SPIKE 2 | Forward: TGC GGC AAG GGC TAC CAC CTG ATG AG | 233 |
Reverse: GGT GTT GTC GGT GGT GAT GAT CTG G | ||
ORI 1A | Forward: GCC TAC ATA CCT CGC TCT GCT AAT C | 110 |
Reverse: GCT GCG CCT TAT CCG GTA ACT ATC | ||
ORI 1B | Forward: AGG CCA CCA CTT CAA GAA CTC T | 78 |
Reverse: CTG GCA GCA GCC ACT GGT A | ||
ORI 2 | Forward: CTA CAT ACC TCG CTC TGC TAA TC | 105 |
Reverse: GCG CCT TAT CCG GTA ACT ATC | ||
KAN 1C | Forward: TCG ACC ACC AAG CGA AAC A | 63 |
Reverse: CGA CAA GAC CGG CTT CCA T | ||
KAN 1S | Forward: GAA ACA TGG CAA AGG TAG CGT T | 93 |
Reverse: TTG ATG GTC GGA AGT GGC ATA A |
Target region | Probe sequence (5’→ 3’) | Fluorescent dye |
|---|---|---|
SPIKE Comirnaty | CCC TCA GTC TGC CCC TCA CGG CGT GGT GTT | Cy5 – BHQ2 |
SPIKE Spikevax | TCC CCA GAG CGC ACC CCA CGG AGT GGT GTT | Cy5 – BHQ2 |
ORI 1 | TAG CAC CGC CTA CAT ACC TCG CTC TGC | HEX – BHQ1 |
ORI 2 | TGC TGC CAG TGG CGA TAA GTC GTG TCT T | FAM – BHQ1 |
KAN 1C | CGC ATC GAG CGA GCA CGT ACT CG | FAM - TAMRA |
KAN 1S | GAT GTT ACA GAT GAG ATG GTC AGA CTA AAC | FAM – BHQ1 |
