Fig. 1: Construction and characterization of recombinant AAV vectors carrying the Der p1-Der p2 fusion protein.
From: The adeno associated viral vectored Dp12S vaccine effective alleviation of asthma symptoms in mice
A Schematic presentation of AAV vectors carrying the Der p1-Der p2 fusion protein. An HA tag and a Flag tag were added to the Napos; terminus of Der p1 and the COOHapos; terminus of Der p2, or expression detection. B Animo acid sequence of Der p1-Der p2 fusion protein: green ‘C’ represents the 12 cysteine residues (dashed vertical lines in A), whereas in Dp12S the cysteine residues were exchanged for serine residues; red ‘S’, and blue AA represent the P2A linker. C The function mechanism of 2A auto-cleavage peptide in the expression of Der p1-Der p2 fusion antigen. D, E The expression of Der p1 and Der p2 by mosaic proteins. SDS-PAGE–containing protein extracts of BEAS-2B expressing Dp12S (D) or HEK293 cells were infected with AAV-Dp12S (S) and a control vector AAV-GFP. Cell culture ‘C’ and the cell lysates ‘L’ were subjected to SDS-PAGE followed by western blot analysis using anti-Dp12S, anti-Der p1, anti-Der p2, and anti-GAPDH antibodies. M: molecular weight markers; C: Cell culture; S: cell lysates. All schematic diagrams (A, C) are prepared using Microsoft PowerPoint.
