Fig. 1: mRNA-LNP vaccine induced IFN reduces overall mRNA uptake by DCs in vitro.
From: Type I interferon restricts mRNA vaccine efficacy through suppression of antigen uptake in cDCs
A (Left) Representation flow plots of eGFP expression of BMDCs incubated with 2.5 μg eGFP mRNA-LNP alone, with mRNA-LNP and MAR1-5A3 antibody (αIFNαR, red), or with mRNA-LNP and 103 U/mL IFNα for 16 h (blue). (Right) eGFP expression as a percentage of all DCs incubated with 2.5 μg eGFP mRNA-LNP alone (white), with mRNA-LNP and MAR1-5A3 antibody (αIFNαR, red), or with mRNA-LNP and 103 U/mL IFNα for 16 h (blue). B (Left) Representative flow plots of CD40 MFI of BMDCs incubated with 2.5 μg eGFP mRNA-LNP alone, with mRNA-LNP and MAR1-5A3 antibody (αIFNαR, red), or with mRNA-LNP and 103 U/mL IFNα for 16 h (blue). (Right) CD40 MFI of DCs incubated with 2.5 μg eGFP mRNA-LNP alone (white), with mRNA-LNP and MAR1-5A3 antibody (αIFNαR, red), or with mRNA-LNP and 103 U/mL IFNα for 16 h (blue). Data represents pooled independent samples from four independent experiments (n = 11 for empty, n = 11 for mRNA alone, n = 12 for IFNα, and n = 11 for αIFNαR). Data were compared using one-way ANOVA.
