Extended Data Fig. 2: Pilot screen using a small subset of the combinatorial library. | Nature Biomedical Engineering

Extended Data Fig. 2: Pilot screen using a small subset of the combinatorial library.

From: Peptide-encoding mRNA barcodes for the high-throughput in vivo screening of libraries of lipid nanoparticles for mRNA delivery

Extended Data Fig. 2

a A list of esters (left) and amines (right) used to generate lipids for the pilot screen. b Peptide barcoding analysis of the pilot library. Only lipids that resulted in a peptide dot product > 0.8 are shown. c Individual particle analysis using the top four hits, showing that the top three lipids from the peptide barcoding analysis do not result in significant protein production. d FLuc assay confirming that FLuc mRNA-containing LNPs composed of any of the three ‘false positives’ alone do not result in detectable protein expression, whereas a 1:1 mixture of hEPO mRNA-containing cKK-e12 LNPs and FLuc mRNA-containing ‘false positive’ LNPs results in robust protein expression. Values shown are the mean of n = 3 mice/group, with error bars representing the SD. The FLuc assay of panel d was performed twice with similar results.

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