Extended Data Fig. 3: A subset of LEPR⁺ cells in the peri-implant fibrotic tissue expresses Cxcl12-GFP.

a, Expression of LepR-tdTomato and Cxcl12-GFP in the peri-implant fibrotic tissue of LepR^cre;Rosa26^tdTomato; Cxcl12^GFP mice underwent fibrous-integrated surgery at postoperative day 14. A subset of LEPR⁺ cells (red) co-localize with cells expressing Cxcl12 (green). Scale bar, 500 μm. b-e, Enlarged view of the outlined blue box of figure in panel a. Scale bar, 50 μm. Images in a-e are representative of at least 3 independent experiments. f, Expression of LepR-tdTomato and Cxcl12-GFP in the peri-implant osseous tissue of LepR^cre;Rosa26^tdTomato; Cxcl12^GFP mice underwent osseointegrated surgery at postoperative day 14. A subset of LEPR⁺ cells (red) in the perivascular area co-localizes with cells expressing Cxcl12 (green). Scale bar, 500 μm. g–j, Enlarged view of the outlined blue box of figure in panel f. Scale bar, 50 μm. Images in f-j are representative of at least 3 independent experiments. k–l, Expression of CXCL12-GFP by Lin-LepR-tdTomato⁺ (k) or osseointegrated surgery (l). m-n, Immunofluorescence quantification of expression of and Cxcl12-GFP in LepR^cre;Rosa26^tdTomato; Cxcl12^GFP mice underwent fibrous-integrated or osseointegrated surgery at postoperative day 14. Data are mean ± s.d. n = 11 for both osseointegrated and fibrous-integrated model. n, Immunofluorescence quantification of co-localization between of LepR-tdTomato and Cxcl12-GFP in LepR^cre;Rosa26^tdTomato; Cxcl12^GFP mice underwent fibrous-integrated or osseointegrated surgery at postoperative day 14. Unpaired, two-tailed Student’s t-test. Data are mean ± s.d. n = 11 for both osseointegrated and fibrous-integrated model. Each dot in m and p corresponds to biologically independent replicates.

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