Extended Data Fig. 5: A subset of LEPR⁺ cells in the peri-implant.

a, Expression of LepR-zsGreen and Acta2-RFP in the peri-implant fibrotic tissue of LepR^cre;Rosa26^Zsgreen; Acta2^mRFP mice underwent fibrous-integrated surgery at postoperative day 14. A subset of LEPR⁺ cells (green) co-localize with cells expressing Acta2 (red). Scale bar, 500 μm. b-e, Enlarged view of the outlined yellow box of the figure in panel a. Scale bar, 50 μm. Images in a-e are representative of at least 3 independent experiments. f, Expression of LepR-zsGreen and Acta2-RFP in the peri-implant osseous tissue of LepR^cre;Rosa26^Zsgreen; Acta2mRFP mice underwent osseointegrated surgery at postoperative day 14. A subset of LEPR⁺ cells (green) in the perivascular area co-localize with cells expressing Acta2 (red). Scale bar, 500 μm. Images in a are representative of at least 3 independent experiments. g–j, Enlarged view of the outlined yellow box of figure in panel f. Scale bar, 50 μm. Images in f–j are representative of at least 3 independent experiments. k–l, Representation of the strategy used for FACS analysis of expression of LepR-zsGreen and Acta2-RFP in Lin-6C3-CD90-CD200⁺ CD105^– fibrous integrated surgery (k) or osseointegrated surgery (l). LepR^cre;Rosa26^Zsgreen; Acta2mRFP (red), LepR^cre;Rosa26^Zsgreen (blue). m-n, Immunofluorescence quantification of expression of LepR-zsGreen and Acta2-RFP in LepR^cre;Rosa26^Zsgreen; Acta2mRFP mice underwent fibrous-integrated or osseointegrated surgery at postoperative day 14. Data are mean ± s.d. n = 5 for both osseointegrated and fibrous-integrated model. Unpaired, two-tailed Student’s t-test. o, Immunofluorescence quantification of co-localization between LepR-zsGreen and Acta2-RFP in LepR^cre;Rosa26^Zsgreen; Acta2mRFP mice underwent fibrous-integrated or osseointegrated surgery at postoperative day 14. Data are mean ± s.d. n = 5 for both osseointegrated and fibrous-integrated model; Unpaired, tailed Student’s t-test. p, Flow cytometry quantification of relative abundance of Lin-LepR-Zsgreen⁺ Acta2-RFP⁺ 6C3^–CD90^– CD200⁺ CD105 in LepR^cre;Rosa26^Zsgreen; Acta2^mRFP mice underwent fibrous-integrated or osseointegrated surgery at postoperative day 14. Data are mean ± s.d. n = 5 for both osseointegrated and fibrous-integrated model; Unpaired, two-tailed Student’s t-test. Each dot in m-p corresponds to biologically independent replicates. Images in k-l are representative of at least 3 independent experiments.

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