Extended Data Fig. 7: Peri-implant fibrosis is persistent up to at least 16 weeks in murine model of peri-implant fibrosis.
a, Micro-computed tomography (μCT) of proximal tibia of LepRcre;Rosa26tdtomato underwent fibrous-integrated surgery at post-operative day 3, day 7, 2 weeks, 4 weeks, 8 weeks, and 16 weeks. Scale bar, 500 μm. b, Hematoxylin and eosin staining of proximal tibia of LepRcre;Rosa26tdtomato underwent fibrous-integrated surgery at post-operative day 3, day 7, 2 weeks, 4 weeks, 8 weeks, and 16 weeks. Scale bar, 500 μm. c, Immunofluorescent imaging proximal tibia of LepRcre;Rosa26tdtomato underwent fibrous-integrated surgery at post-operative day 3, day 7, 2 weeks, 4 weeks, 8 weeks, and 16 weeks. Scale bar, 500 μm. Bottom row, enlarged view of the outlined yellow box at each time-point. Scale bar, 50 μm. d, Bone volume/total volume (BV/TV)) of mice at post-operative day 3, day 7, 2 weeks, 4 weeks, 8 weeks, and 16 weeks. Data are mean ± s.d. n = 6. e, Histology quantification of peri-implant fibrosis (% Fibrosis) and peri-implant bone (% bone) of mice at at post-operative day 3, day 7, 2 weeks, 4 weeks, 8 weeks, and 16 weeks. Data are mean ± s.d. n = 6. f, Immunofluorescent quantification of peri-implant fibrosis (% Fibrosis) and peri-implant bone (% bone) of mice at at post-operative day 3, day 7, 2 weeks, 4 weeks, 8 weeks, and 16 weeks. Data are mean ± s.d. n = 6. Each dot in d-f corresponds to biologically independent replicates. Images in a–c are representative of at least 3 independent experiments.
