Extended Data Fig. 10: Administration of neutralizing antibody against reduces LepR– tdTomato+ cells but does not affect Lin– 6C3– CD90– CD200+ CD105– (mSSC) subpopulation.
a, Immunofluorescent imaging of CD200-antibody stained. proximal tibia of LepRcre;Rosa26tdtomato receiving either anti-ADGRF5 or isotype control as prophylaxis. Scale bar, 500 μm. Right column, enlarged view of the outlined yellow box. Scale bar, 50 μm. b, Immunofluorescent imaging of CD200-antibody-stained proximal tibia of LepRcre;Rosa26tdtomato receiving either anti-ADGRF5 or isotype control as treatment starting from postoperative day 14. Scale bar, 500 μm. Right column, enlarged view of the outlined yellow box. Scale bar, 50 μm. c,f FACS analysis of TdTomato+ subset of Lin-6C3– CD90–CD200+ CD105– (TdTomato+ mSSC) in mice group receiving anti-ADGRF5 as compared to isotype control, either as prophylaxis (c) or as treatment (f). Anti-ADGRF5 (n = 5), isotype control (n = 5). Unpaired, two-tailed Student’s t-test. Data are mean ± s.d. d,g, FACS analysis of Lin– 6C3– CD90– CD200+ CD105– (mSSC) between mice receiving daily anti-ADGRF5 or isotype control either as prophylaxis (d) or as treatment (f). Anti-ADGRF5 (n = 5), isotype control (n = 5). ns= not significant. Unpaired, two-tailed Student’s t-test. treatment (f). Anti-ADGRF5 (n = 5), isotype control (n = 5). Unpaired, two-tailed Student’s t-test. Data are mean ± s.d. e, Host bone-implant failure load of LepRcre;Rosa26tdtomato receiving either daily anti-ADGRF5 or isotype control as prophylaxis at postoperative day 28. n = 5 for mice receiving anti-ADGRF5 and n = 5 for mice receiving isotype control. Unpaired, two-tailed Student’s t-test. Data are mean ± s.d. h, Host bone-implant failure load of LepRcre;Rosa26tdtomato receiving either daily anti-ADGRF5 or isotype control as treatment starting from postoperative day 14 and evaluated at postoperative day 28. n = 5 for mice receiving anti-ADGRF5 and n = 5 for mice receiving isotype control. Unpaired, two-tailed Student’s t-test. Data are mean ± s.d. Images in a-b are representative of at least 3 independent biological replicates. Each dot in c–e and f–h corresponds to biologically independent replicates.
