Extended Data Fig. 2: Increased memory immune responses elicited by iPM nanovax. | Nature Biomedical Engineering

Extended Data Fig. 2: Increased memory immune responses elicited by iPM nanovax.

From: Potent prophylactic cancer vaccines harnessing surface antigens shared by tumour cells and induced pluripotent stem cells

Extended Data Fig. 2: Increased memory immune responses elicited by iPM nanovax.

The healthy C57BL6N mice received four rounds of iPM nanovax vaccination. Two months later, flow cytometry analysis was performed to determine the percentage of memory B cells and T cells in mice. The frequency of plasma cells (CD3−CD19−CD138−IgM+) in peripheral blood (a) was detected. The percentage of plasma cells (CD3−CD19−CD138−IgM+) and plasmablast cells (CD3−CD19+CD138+IgM+) in bone marrow (b) were detected. The frequency of memory B cells (CD3−CD19+CD138−PDL2+IgM+) in bone marrow (c), central memory T cells (CD44highCD62LhighCD8+ and CD44highCD62LhighCD4+) in peripheral blood (d) and spleen (e) were detected. The data are presented as the mean ± s.d. (n = 6 biological replicates). P values in a–e were calculated using an unpaired t-test.

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