Extended Data Fig. 1: Liposomes promote circRNA delivery and antigen presentation in DCs.
From: Small circular RNAs as vaccines for cancer immunotherapy
a, DLS data showing the size distribution of blank liposome and lipo-circRNA with different N/P ratios in PBS (solid) and 1% FBS (dotted). b, Zeta potential of blank liposome and lipo-circRNA. c-d, In vitro cell uptake of circRNA and antigen presentation in DCs mediated by lipo-circRNA. c, Flow cytometry results and d, MFI of the SIINFEKL presentation in SIINFEKL-circRNA-treated DC2.4 cells (24 h). SIINFEKL antigen presentation on DC2.4 cells that were treated with Lipofectamine 3000-transfected circRNA and lipo-circRNA with different N:P ratios, respectively. Ns: non-significant, *p < 0.05, ***p < 0.001, by one-way ANOVA with Bonferroni post-test. e-h, In vitro intracellular delivery of circRNA in DCs by liposome. e, Confocal microscopy images of DC2.4 cells treated with lipo-circRNA for 1 h, 3 h, or 6 h. Blue: nuclei stained with Hoechst33342. Green: endolysosome stained with LysoTracker Green DND-26. Red: Cy5-circRNA. Insets: close-up views of single cells. f, Flow cytometry results of DC2.4 cells incubated with Lipo-circRNA and free circRNA for different times. g, Flow cytometry results showing MFI of DCs incubated with free or Lipo-Cy5-circRNA. ****p < 0.0001, by t-test of the AUC. h, The signal ratio of Cy5-circRNA outside/inside (O/I) the endolysosome.
