Extended Data Fig. 2: Representative imaging analysis of KIM-1+ tubules and proximal tubuli in renal tissue.

(a) Representative immunofluorescence staining of KIM-1+ tubules in renal tissue sections after 24 h different treatments. Higher-magnification images of the KIM-1+ tubules in renal tissue sections are shown at the bottom, and renal tubules were stained with anti-KIM-1 antibody and FITC-conjugated secondary antibody (green: KIM-1; blue: DAPI; wireframe indicates the magnified image). Scale bars, 500 μ (top) and 50 μm (bottom). (b) Representative fluorescence imaging (green: wheat germ agglutinin (WGA) lectin; blue: DAPI) and scanning electron microscopy (SEM) micrographs of proximal tubuli in mice after 24 h different treatments. The renal tubuli were labelled with FITC-conjugated-WGA lectin. Scale bars, 10 μm (top) and 5 μm (bottom).