Extended Data Fig. 2: Cas12a-mediated somatic genome editing induces indels at target genetic loci, while incorporation of Cas12a-mediated genome editing with Tuba-seq^Ultra enables quantification of the effects of each crRNA array.

a-c. Indel frequencies for genomic regions targeted by each crRNA within bulk tumour-bearing lung tissue (a), microdissected lung tumours (b) or bulk tumour-bearing pancreas tissue (c) from H11^LSL-Cas12a mice. Tumours were initiated with the indicated lentiviral vector pool. Pancreas tissue samples (c) had variable tumour burden, likely explaining observed variability in indel frequencies among samples. Percentages shown indicate the presence of Cas12a-mediated indels, rather than the absolute measurement of Cas12a or individual crRNA efficiency. For a detailed assessment of the efficiency of Cas12a-mediated editing in vivo, see Fig. 6 and Extended Data Figs. 6–8. Symbol shapes and colours identify values from the same sample. Bars represent means. d. Detailed schematic of tumour barcoding with high-throughput BC-crRNA sequencing to determine the size of each Cas12a-induced clonal tumour. e-f. Number of tumours (e) and mean tumour size assuming a log-normal distribution (f) for each crRNA array (27 combinations in total) relative to the median value for all arrays in H11^LSL-Cas12a mice. Medians and 95% confidence intervals are shown based on bootstrap resampling with 1,000 iterations.