Extended Data Fig. 4: Generation of Cas12a Lenti-U6BC-crRNA-Cre vector libraries is associated with minimal shuffling of crRNA sequences.
From: Efficient and multiplexed somatic genome editing with Cas12a mice
a,c,e. Fraction of all U6-integrated barcode reads that are associated with only a single crRNA array within each vector pool in the indicated mouse genotypes: Lenti-U6BC-crNf1/Rasa1/Pten-Cre (a), Lenti-U6BC-crRb1/Trp53/Rbl2-Cre (c), and Lenti-U6BC-crTrp53/Cdkn2a/Smad4-Cre (e). Each dot represents a mouse. Bars represent medians. b,d,f. Fraction of total neoplastic cells (total tumour burden) remaining after filtering out spurious “tumour” reads arising during library preparation and sequencing of each pool in the indicated mouse genotypes: Lenti-U6BC-crNf1/Rasa1/Pten-Cre (b), Lenti-U6BC-crRb1/Trp53/Rbl2-Cre (d), and Lenti-U6BC-crTrp53/Cdkn2a/Smad4-Cre (f). Each dot represents a mouse. Bars represent medians.
