Fig. 1: Schematic of fuzzy sequencing.
From: A fuzzy sequencer for rapid DNA fragment counting and genotyping
a, Chemical structure of the TPLFN used in fuzzy sequencing. b, Schematic of the fluorogenic sequencing chemistry adopted by fuzzy sequencing. CIP, calf intestinal alkaline phosphatase. c, Schematic of the fuzzy sequencing pipeline. The DNA templates are amplified on the beads and loaded into the wells of the sequencing chip. After hybridization by the sequencing primers, the DNA templates are primed for sequencing. d–f, Schematic of the nucleotide flowgrams used in single-nucleotide addition (d), monochromatic double-nucleotide addition (BitSeq; e) and dichromatic double-nucleotide addition (SuperBitSeq; f).
