Extended Data Fig. 8: Chemical synthesis and characterization of Peking Orange (PO).
From: A fuzzy sequencer for rapid DNA fragment counting and genotyping
(a) The synthetic route of the fluorophore PO. (b–i) NMR of intermediate products. (b, c) Chemical 1a. (d, e) Chemical 1b. (f, g) Chemical 1c. (h, i) Chemical PO. (bdfh) 1H-NMR. (cegi) 13C-NMR. (j) HRMS of PO. (k) Excitation and emission spectra of PO. (l) The fluorescence emission of PO is pH sensitive. The pink shade indicates the standard deviation. (m) Fluorogenic kinetics of PO-dN4P extending multiple bases. (n) The sequencing reaction kinetics using PO-dN4P and TG-dN4P as substrates.
