Extended Data Fig. 5: Selectivity studies with DEL-derived hits by fluorescence polarization. | Nature Biomedical Engineering

Extended Data Fig. 5: Selectivity studies with DEL-derived hits by fluorescence polarization.

From: Discovery of high-affinity ligands for prostatic acid phosphatase via DNA-encoded library screening enables targeted cancer therapy

Extended Data Fig. 5: Selectivity studies with DEL-derived hits by fluorescence polarization.The alternative text for this image may have been generated using AI.

In comparison to (a) benchmark compound s56, DEL-derived-compounds (b) ProX2-(S)-Fluo (s27a), (c) ProX2-(R)-Fluo (s27b), (d) ProX1-(SS).Fluo (8), (e) ProX3-(S)-Fluo (9), (f) ProX3-(R)-Fluo (s33) presented >50-fold improvement of binding affinity. The molecules presented no-cross reactivity with alternative phosphatases (PLAP and TNAP) and bound to albumins (HSA and MSA) in the micromolar concentration range. Final compound concentration used corresponds to 5 nM for test items. Data is plotted as mean, with error bars indicating standard deviations of the replicates (n = 3, except for PLAP and TNAP in (d) where n = 2).

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